![]() Expression pattern analysis of OsNRT2.3 and its co-expressed genes in tissue-specific and nitrogen (N) response led to the discovery of OsAMT1.3, OsZIFL9, OsbZIP27, and OsIRT1 as four strong candidates to participate in drought stress during NO 3 - uptake system. Furthermore, half of the co-expressed genes were enriched in nitrogen use efficiency (NUE)-related processes, such as transport, stress, macromolecule metabolic pathways, and transcription regulation. Interestingly, co-expression analysis revealed that potential drought-related genes were expressed similarly to the functional NUE gene, OsNRT2.3. Phylogenetic and motifs analysis clustered genes encoding NRT2 proteins into four monophyletic groups, and the motifs of NRT2 genes were significantly conserved for the specific domain of NO 3 - transmembrane transporter. This study conducted an in silico analysis on 20 NRT2 family genes of rice, wheat, soybean, barley, maize, and papaya. Although several NRT genes are identified and characterised in plants, knowledge of the NRT2 gene family and its nitrogen use efficiency (NUE) function in drought stress has remained elusive in rice. Nitrate transporter (NRT) is responsible for the molecular mechanism of the root nitrate (NO 3 -) uptake system for plant development. ![]()
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